The school is divided in two sessions:

  • Session I: “From Gene to Crystal”, 21-24 January 2020 (max 12 participants) this session is mainly devoted to chemists and small molecule crystallographers)
  • Session II: “From Crystal to Structure”, 3-6 March 2020 (max 15 participants) this session is mainly devoted to biologists and chemists)

You can apply for one or both sessions (the subscription fee shown in the registration page covers one session) 캐드마스 무료 다운로드.

Session I
From Gene to Crystal

21st January 2020

12:00-16.30 Participant registration
14.30-15.00 Welcome by Organizing Committee
15.00-16.00 OPENING LECTURE (M encyclopedia. Bolognesi, UniMI)
past, present, and future

16.00-18.00 Sponsor talks

22nd January 2020

Topics of the day
Cloning and protein expression

09:00-10:00 THEORETICAL LECTURE (P ebs 인터넷 강의 다운로드. Storici, Sbl-Elettra)
Protein expression in eukaryotic systems
10:20-11:00 TECHNICAL LECTURE (S. Onesti, Sbl-Elettra)
Bioinformatics analysis
11:00-11:40 TECHNICAL LECTURE (M 원랜디 6.1 다운로드. Semrau, Sbl-Elettra)
Cloning and site directed mutagenesis
11:40-12:30 TECHNICAL LECTURE (R. Caliandro, UniBZ)
Expression by prokaryotic systems
Primer design
Protein expression in E.coli

23rd January 2020

Topic of the day
Purification techniques for protein

09:00-10:00 THEORETICAL LECTURE (B outer tales 다운로드. Giabbai, Sbl-Elettra)
Protein purification strategies
10:20-11:00 TECHNICAL LECTURE (G. Tassone, UniSI)
Chromatographic techniques
11:00-11:40 TECHNICAL LECTURE (C 한글 2017 무료 다운로드. Pozzi, UniSI)
Co-expression systems
11:40-12:30 TECHNICAL LECTURE (M. Mazzorana, Diamond)
Protein quality control for structural biology
Protein purification by affinity chromatography
Protein purification by size exclusion chromatography

24th January 2020

Topics of the day
Protein crystallization and crystal handling

09:00-10:00 THEORETICAL LECTURE (D far cry 4 다운로드. Lamba, CNR-IC)
Crystallization: how it works
10:20-11:00 TECHNICAL LECTURE (D. Lamba, CNR-IC)
Crystallization techniques
11:00-11:40 TECHNICAL LECTURE (M Download the consolas font. Mazzorana, Diamond)
Membrane protein crystallization
11:40-12:30 TECHNICAL LECTURE (B.D. Belviso, Diamond)
Crystal handling
Solubility test for crystallization
Vapor diffusion crystallization

Session II
From Crystal to Structure

22nd September 2020

12:00-16.30 Participant registration
14.30-15.00  Welcome by Organizing Committee
15.00-16.00 OPENING LECTURE (R tcpmon 다운로드. Caliandro/ D. Lamba, CNR-IC)
Next generation of crystallographers:
background and new challenges
16.00-18.00 Sponsor talks

23rd September 2020

Topics of the day
Crystallographic data collection

BEAMLINE XRD2 AND LABS (A 킹스맨2 자막 다운로드. Heroux and MX staff)
this session will be held at XRD2 beamline and related crystal preparation labs and will cover the following topics:
– X-ray diffraction data collection at beamline
– Crystal preparation for diffraction experiment
– Synchrotron beamline
– Data collection and data collection optimization

24th September 2020

Topics of the day
Data analysis, reduction and phasing

09:00-10:00 THEORETICAL LECTURE (R. Caliandro, CNR-IC)
Analysis of diffraction data
10:20-11:00 TECHNICAL LECTURE (N. Demitri, Elettra)
Data reduction
11:00-11:40 TECHNICAL LECTURE (S. Onesti, Sbl-Elettra)
Phasing methods
11:40-12:30 TECHNICAL LECTURE (M. Mazzorana, Diamond)
Map improvement strategies
Data reduction (XDS, mosflm, xia2)
phasing by Molecular Replacement
experimental phasing

25th September 2020

Topics of the day
Refinement process, validation, deposition and molecular visualization

09:00-10:00 THEORETICAL LECTURE (M. Mazzorana, Diamond)
What can you do with your PDB file?
10:20-11:00 TECHNICAL LECTURE (B.D. Belviso, CNR-IC)
Using CCP4 and PHENIX for structural refinement
11:00-11:40 TECHNICAL LECTURE (D. Lamba, CNR-IC)
Validation process
11:40-12:30 TECHNICAL LECTURE (B.D. Belviso, CNR-IC)
Protein visualization by Pymol and Coot
Refinement process
Molecular graphics with Python and Coot